Analysis of Polymorphism of 18S rRNA Gene in Wuchereria bancrofti Microfilariae
Identifieur interne : 008552 ( Main/Exploration ); précédent : 008551; suivant : 008553Analysis of Polymorphism of 18S rRNA Gene in Wuchereria bancrofti Microfilariae
Auteurs : Yashodhar Bhandari [Inde] ; Pankaj Dabir [Inde] ; Krithika Nandhakumar [Inde] ; Kannayakanahalli Maheshwarappa Dayananda [Inde] ; Yogesh Shripad Shouche [Inde] ; Maryada Venkata Rami Reddy [Inde]Source :
- Microbiology and Immunology [ 0385-5600 ] ; 2005-10.
Abstract
The polymorphism of the 18S rRNA gene in Wuchereria bancrofti microfilariae (mf) collected from three different zones in India was analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The RFLPs of the amplified products obtained after digestion with restriction enzymes SspI, MspI and HhaI showed no difference in the banding patterns among the mf isolates from different endemic zones. Further the sequencing of PCR products did not show any difference in the nucleotide sequence either. The phylogenetic analysis of the sequences of W. bancrofti mf isolates from different endemic zones has shown branching with the earlier reported sequences of W. bancrofti and its close relative Brugia malayi.
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DOI: 10.1111/j.1348-0421.2005.tb03682.x
Affiliations:
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The RFLPs of the amplified products obtained after digestion with restriction enzymes SspI, MspI and HhaI showed no difference in the banding patterns among the mf isolates from different endemic zones. Further the sequencing of PCR products did not show any difference in the nucleotide sequence either. The phylogenetic analysis of the sequences of W. bancrofti mf isolates from different endemic zones has shown branching with the earlier reported sequences of W. bancrofti and its close relative Brugia malayi.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Bhandari, Yashodhar" sort="Bhandari, Yashodhar" uniqKey="Bhandari Y" first="Yashodhar" last="Bhandari">Yashodhar Bhandari</name></noRegion><name sortKey="Dabir, Pankaj" sort="Dabir, Pankaj" uniqKey="Dabir P" first="Pankaj" last="Dabir">Pankaj Dabir</name><name sortKey="Dayananda, Kannayakanahalli Maheshwarappa" sort="Dayananda, Kannayakanahalli Maheshwarappa" uniqKey="Dayananda K" first="Kannayakanahalli Maheshwarappa" last="Dayananda">Kannayakanahalli Maheshwarappa Dayananda</name><name sortKey="Nandhakumar, Krithika" sort="Nandhakumar, Krithika" uniqKey="Nandhakumar K" first="Krithika" last="Nandhakumar">Krithika Nandhakumar</name><name sortKey="Reddy, Maryada Venkata Rami" sort="Reddy, Maryada Venkata Rami" uniqKey="Reddy M" first="Maryada Venkata Rami" last="Reddy">Maryada Venkata Rami Reddy</name><name sortKey="Reddy, Maryada Venkata Rami" sort="Reddy, Maryada Venkata Rami" uniqKey="Reddy M" first="Maryada Venkata Rami" last="Reddy">Maryada Venkata Rami Reddy</name><name sortKey="Shouche, Yogesh Shripad" sort="Shouche, Yogesh Shripad" uniqKey="Shouche Y" first="Yogesh Shripad" last="Shouche">Yogesh Shripad Shouche</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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